LIFA熒光壽命系統附件
簡 述：
LIFA是適用于熒光壽命顯微成像（FLIM）。該設備可以利用頻域技術在任何寬場顯微熒光成像系統獲得壽命變化相關圖像。系統包含TRiCAM?M可調制型像增強CCD相機，可調制光源，調制控制器，定制電腦以及LI-FLIM軟件。像增強相機包含像增強器，CCD相機，可以獲得*高增益和采集速率。
品 牌：
荷蘭 Lambert Instruments
產品型號：
- LIFA
LIFA熒光壽命系統附件
LIFA是適用于熒光壽命顯微成像（FLIM）。該設備可以利用頻域技術在任何寬場顯微熒光成像系統獲得壽命變化相關圖像。系統包含TRiCAM M可調制型像增強CCD相機，可調制光源，調制控制器，定制電腦以及LI-FLIM軟件。像增強相機包含像增強器，CCD相機，可以獲得*高增益和采集速率。
LIFA熒光壽命系統附件關鍵特點：
精準的壽命成分：0-300ns的熒光壽命，精度可達30ps RMS
系統技術參數：
控制器參數：控制器可以經USB線LI-FLIM軟件控制
多LED光源
多激光光源
TRICAM M相機像增強器指標
CCD相機指標
LIFA是適用于熒光壽命顯微成像（FLIM）。該設備可以利用頻域技術在任何寬場顯微熒光成像系統獲得壽命變化相關圖像。系統包含TRiCAM M可調制型像增強CCD相機，可調制光源，調制控制器，定制電腦以及LI-FLIM軟件。像增強相機包含像增強器，CCD相機，可以獲得*高增益和采集速率。
LIFA熒光壽命系統附件關鍵特點：
精準的壽命成分：0-300ns的熒光壽命，精度可達30ps RMS
系統技術參數：
| Lifetime range | 0 - 300 ns |
| Lifetime resolution | less than 100 ps (single pixel) |
| Speed | 2 lifetime images per second (full frame) |
| Spatial resolution | 26 lp/mm (Gen II); 19 lp/mm (Gen III) |
| Sensitivity | single photon |
| Intrascene dynamic range | 12 bit |
| Camera input format | 1 inch |
| Effective pixel size | 10.3 μm square (12,9 for lens coupled CCD) |
| Signal shape | Sinusoidal |
| Frequency range | 1 MHz – 120 MHz |
| Frequency adjustment resolution | 1 kHz |
| Frequency deviation | 100 ppm max. |
| Harmonics suppression | Better than 25 dB |
| Output 1 Type a: MultiLED AC signal level DC current adjust |
Capable of driving lightsource type a, optionally b and c Adjustable in 250 steps 0 – 1000 mA, 12 bit resolution |
| Optional lightsource outputs: Type b: Modulated laser diode AC signal level DC level adjust Type c: AOM for CW laser AC signal level Frequency Output 2 AC signal level AC signal after amplification |
0.00 – 1.00 V peak-peak 0.00 – 1.00 V 0.00 – 1.00 V peak-peak Half the frequency of output 2 To image intensifier Adjustable from –50 to 0 dBm 15 Vpp nom. |
| External trigger input | LVTTL (phase shift on falling edge or rising edge) |
| Impedance | 50 Ω |
| Phase adjustment range | 0 - 360° in 1° steps |
| Phase deviation | < 0.01° |
| Cathode voltage DC | Adjustable from –150 V to +15 V |
| MCP voltage (gain) DC | Adjustable from 450 – 1000 V max. |
| Anode voltage DC | Fixed 6 kV |
| Peak wavelengths (nm), approximately | 405 (CFP), 445 (cerulean, CFP), 470 (GFP), 535 (TRITC), 630 (CY5), others on request |
| Number of mounted LEDs | 3 (standard), or 4 (maximum) |
| Modulation frequency | up to at least 80 MHz |
| LEDs switching time | 200ms |
| Coupling | Compatible with existing Hg and Xe microscope adapters |
| Lifetime drift | less than 30 ps* |
| Optional Temperature stabilization for long-term (hours) lifetime stability |
Active thermoelectric water cooling/heating system |
| Control temperature range | 5 – 45º C |
| Temperature stability (constant load, constant ambient temp) |
± 0.1º C |
| Dimensions (l x w x h) | 346 x 109 x 183 mm |
| * Measured using stable fluorescent material with single lifetime component of 3.2 ns | |
| Wavelengths (nm) | 375 (BFP), 405 (CFP), 445 (cerulean, CFP), 488 (GFP), 515 (YFP, Venus), 647 (CY5), others on request |
| Number of laser lines | Standard: up to 3 or up to 6; Extreme: up to 4. All combined into one optical fiber output |
| Output power (mW) | 20 mW-300 mW, depending on the wavelength |
| Modulation frequency | Up to 120 MHz (standard) |
| Input signal | Digital: 0...1 V / 50 Ω |
| Control interface | RS232 |
| Coupling | KineFLEX system, with a single-mode polarization maintaining fiber and either FC-APC, FC-PC or FC-P8 output connector |
| Power stability | Standard: 2%*, Extreme: 0.5%* |
| Lifetime drift | Standard: less than 50 ps*, Extreme: less than 20-30 ps* |
| Dimensions (l x w x h) | 19" rack housing. MultiLASER-3: 480 x 484 x 88 mm; MultiLASER-4 and 6: 610 x 484 x 132 mm |
| Options | • Multi-channel excitation • Two single-mode optical fiber outputs • Custom fiber outputs on special request |
| * Measured using stable fluorescent material with single lifetime component of 3.2 ns | |
CCD相機指標
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